Procaspase-Activating Compound-1 Synergizes with TRAIL to Induce Apoptosis in Established Granulosa Cell Tumor Cell Line (KGN) and Explanted Patient Granulosa Cell Tumor Cells In Vitro
نویسندگان
چکیده
Granulosa cell tumors (GCT) constitute only ~5% of ovarian neoplasms yet have significant consequences, as up to 80% women with recurrent GCT will die the disease. This study investigated effectiveness procaspase-activating compound 1 (PAC-1), an activator procaspase-3, in treating adult (AGCT) combination selected apoptosis-inducing agents. Sensitivity AGCT line KGN these drugs, alone or PAC-1, was tested using a viability assay. Our results show wide range cytotoxic activity among agents tested. Synergy PAC-1 most pronounced, both empirically and by mathematical modelling, when combined tumor necrosis factor-related ligand (TRAIL). showed rapid kinetics apoptosis induction determined caspase-3 activity, strongly synergistic killing well patient samples primary AGCT. We demonstrated that novel two pro-apoptotic agents, TRAIL significantly amplified cells, warranting further investigation this potential therapy for
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2021
ISSN: ['1661-6596', '1422-0067']
DOI: https://doi.org/10.3390/ijms22094699